Genomics, Epigenomics and Sequencing Core (GES Core)
Xiang Zhang, PhD; Core Director & Assoc. Prof.
The Genomics, Epigenomics and Sequencing Core (GES Core), originally named Genomics and Microarray Laboratory, was established in 1999. In 2012, the Core was transformed into the next generation sequencing-based facility, which provides services to researchers at the University of Cincinnati, Cincinnati Children's Hospital Medical Center as well as other institutions nationwide. Since then, the Core has been fully sustainable and always maintains financially healthy status. We now have one of the best equipped laboratories in the College of Medicine for the next generation sequencing, single cell sequencing and many other services in genomics and epigenomics.
GES Core uses UC Stratocore for sample submission and billing. Here are the related links:
Placing an order via UC Stratocore (required): https://ppms.us/uc/start/
How to use UC Stratocore (important): https://med.uc.edu/depart/eh/cores/genomics/services-and-order
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RNA/DNA extraction.
Service rate is depdending on sample type and number.
Cost:Request Information$40/sample.
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10x Genomics single cell sequencing.
Cost:Request InformationPlease inquire.
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DNA/RNA Bioanalyzer QC (measure sample size distribution and integrity).
Cost:Request Information$25
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miRNA-seq (standard service; PE2x61 bp, ~10M reads/5M read clusters, >3M pass filter reads).
Cost:Request Information$195
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PolyA RNA-seq (standard service; PE2x61 bp, ~60M reads/30M read clusters, >40M pass filter reads)
Cost:Request Information$230
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Other types of sequencing (varies with experiment approach, kit/reagent, sequencing setting, etc).
Cost:Request InformationPlease inquire.
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MethylationEPIC and Genotyping.
Cost:Request InformationPlease inquire.
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Other types of services (supportive DNA/RNA experiments).
Cost:Request InformationPlease inquire.
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Note 1: The rates are approved by UC Recharging Meeting. Due to the price change of kits, reagents, consumables, etc., they are subject to change without prior notice.
Cost:Request InformationFor inquiry, please email xiang.zhang@uc.edu.
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Note 2: Collaboration and/or co-authorship maybe required if the Core has significant intellectual contribution.
Request Information
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GES Core major equipment
Request Information
GES Core has three Revco high-performance ultra-low freezers, four Revco high-performance freezers, and three refrigerators, which are equipped with Smart-Vue Sensors to monitor and report temperature change in real-time via wireless connection to cell phones and emails. The Core is equipped with a series of instruments to support its service and research, from DNA/RNA isolation to library preparation and sequencing. This includes Illumina NextSeq 2000 sequencer and all the ancillary instruments including:
ABI QuantStudio 5 Real-Time PCR System for library quantification, gene expression, genotyping and CNV analyses, Covaris S2 for precise size control of DNA fragmentation, Eppendorf Thermomixer block shakers with heating and cooling (two), 4X microplates shaker (two) incubators (two), Agilent Bioanalyzer and Invitrogen Qubit fluorometers (two) for DNA, RNA and library QC, Veriti and VeritiPro thermal cyclers (total 8) for enzyme reactions and DNA amplification, Labconco entriVap micro IR Vacuum Concentrator for DNA/RNA samples, and Labnet ENDURO GDS Touch Gel Documentation System agarose/PAGE gel electrophoresis systems for library/DNA/RNA analysis and size selection. It has a total of 26 different types of centrifuges to meet different experiment requirements. In addition, it is equipped with MPBio FastPrep-24 5G homogenizer for high quality RNA extraction from all types of samples. The Core has Illumina iScan and its ancillary instruments used in Infinium BeadChip System. Further, the Core has six different types of Schneider Electric Smart-UPS connected to its critical instruments to prevent potential power failure.
For single cell sequencing, GES Core has 10x Genomics Chromium Single Cell System and its supportive instruments such as LUNA-FL Automated Fluorescence Cell Counter and fully automated gentleMACS Octo Dissociator with Heaters from Miltenyi.
GES Core values the role of lab automation in its service. It continuously invests in the Integra automated pipetting system and has developed many validated apps for large experiments. Combined with the access to NovaSeq X Plus sequencer, the Core efficiently processes large numbers of samples routinely in a cost-effective manner.