Terry L Kirley , PhD

Education

BS in Biology, PhD in Chemistry, and post-doctoral training in Pharmacology

Research

Structural characterization and functional analysis of an anti-cocaine monoclonal antibody for treatment of cocaine addiction 

My laboratory is currently collaborating on a therapeutic anti-cocaine antibody project with the laboratory of Dr. Andrew Norman.  We have characterized the post-translational modifications and analyzed the heterogeneity of this monoclonal antibody, as well as developed methods for generation and purification of antibody fragments, including the therapeutically relevant Fab fragment.  We also developed fluorescence quenching methods to measure binding affinities of the antibody and its fragments for cocaine and its metabolites and derivatives.  We are currently characterizing the stability of the intact antibody, crystalizing the Fab fragment, and determining the protein and glycan heterogeneities and post-translational modifications of this monoclonal antibody to seek FDA approval for human clinical trials.
           
Another long-standing goal of the laboratory has been the determination of the structures and functions of the cell membrane and soluble ecto-nucleotidases. One family of these enzymes are collectively called ecto-Nucleoside Triphosphate Diphosphohydrolases (eNTPDases), and another is a structurally unrelated nucleotidase is called SCAN (Soluble Ca-Activated Nucleotidase).  The ecto-nucleotidases are important for the regulation of many physiological and pathological signaling processes under purinergic signaling control, including pain perception and maintenance of hemostasis via hydrolysis of the platelet activator, ADP.

Related to the nucleotidases, I also have ongoing collaborations with Dr. Peter Roach (Indiana University) using the SCAN nucleotidase to characterize glycogen phosphorylation, and with Dr. Neil Ayres (Department of Chemistry, here at UC), testing the suitability of coatings his laboratory synthesizes for making materials biocompatible with blood.

Bachelor's Degree: University of Cincinnati Cincinnati, OH, 1978 (Biology)

Master's Degree: University of Cincinnati Cincinnati, OH, 1980 (Chemistry)

Doctoral Degree: University of Cincinnati Cincinnati, OH , 1982 (Biochemistry)

Postdoctoral Degree: University of Cincinnati Cincinnati, OH, 1985 (Pharmacology and Cell Biophysics)

Antibody Utilization, Characterization, Fragmentation, and Structure.
Purinergic Signaling, Extracellular Nucleotides and Nucleotidases.
Enzyme Analysis and Structure, Enzyme Inhibitors.
Protein Expression and Refolding, Protein Engineering.
Cysteine and Disulfide Chemistry and Analysis.
Ligand Binding Techniques and Applications. 

1985 -1993 Assistant Professor, Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine ,

1993 -2004 Associate Professor (with tenure), Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine ,

2004 -To Present Professor, Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine ,

1986 -1997 Member of the Protein Society , Protein Society ,

1984 -1995 Member of the Biophysical Society , Biophysical Society ,

1991 -1996 Member of American Association for the Advancement of Science , American Association for the Advancement of Science ,

2003 -To Present Member of American Association for the Advancement of Science , American Association for the Advancement of Science ,

1980 -1992 Member of the American Chemical Society , American Chemical Society ,

Grant: #NIH R01 HL72382 Investigators:Terence L. Kirley 07-14-2003 -05-31-2007 Expression and Structure of Soluble Human Apyrases Role:PI Active

Grant: #NIH R01 HL72382-S1 Investigators:Terence L. Kirley 08-01-2005 -05-31-2007 Expression and Structure of Soluble Human Apyrases Role:PI Active

Grant: #NIH R01 HL59915 Investigators:Terence L. Kirley 02-01-2000 -01-31-2005 "Active Site Determination of the E-type ATPases" Role:PI Completed

Grant: #NHLBI Shared Research Facility for Molecular Biology Investigators:Jerry B. Lingrel Role:PI Completed

Grant: #Training Grant Investigators:Arnold Schwartz 07-01-1988 -06-30-1996 "Training in Cardiovascular Biology" Role:PI Completed

Grant: #SW-88-24 Investigators:Terence L. Kirley 07-01-1988 -06-30-1989 Ohio Affiliate, American Heart Association "Identification of Functionally Essential and Structurally Important Amino Acids in (Na,K)-ATPase" Role:PI Completed

Investigators:Terence L. Kirley 01-01-1988 -12-31-1988 University Research Council Faculty Research Support "Identification of Functionally Essential and Structurally Important Amino Acids in (Na,K)-ATPase" Role:PI Completed

Grant: #SW-87-27 Investigators:Terence L. Kirley 07-01-1987 -06-30-1988 Southwestern Ohio Chapter, American Heart Association "Isolation and Characterization of Transverse Tubule Mg-ATPase" Role:PI Completed

University Research Council Faculty Research Support "Identification and Characterization of the Mg-ATPase Activity in Rabbit Skeletal Muscle Transverse Tubules" Completed

Grant: #SW-86-15 Investigators:Terence L. Kirley 07-01-1986 -06-30-1987 Southwestern Ohio Chapter, American Heart Association Structural Characterization of Transverse Tubule Mg-ATPase Role:PI Completed

Grant: #NIH P01-HL22619 Investigators:Arnold Schwartz 07-01-1986 -06-30-1988 Supplement to Protein Chemistry Core Facility Cellular Mechanisms of Cardiac Failure Role:pi Completed

Grant: #NIH P01-HL22619 Investigators:Arnold Schwartz 07-01-1988 -06-30-1993 Core 2 - Protein Chemistry Core Cellular Mechanisms of Cardiac Failure Role:PI Completed

Grant: #NIH RO1 AR38576 Investigators:Terence L. Kirley 03-01-1989 -02-28-1994 Characterization of Transverse Tubule Mg-ATPase Role:PI Completed

Grant: #NIH: RCDA Investigators:Terence L. Kirley 07-01-1991 -06-30-1996 Characterization of Transverse Tubule Mg-ATPase Role:PI Completed

Investigators:Richard A. Walsh Role: Coordinator 01-01-1990 -12-31-1990 RCP: Research Challenge Program Completed

Investigators:Terence L. Kirley 1990 -1992 RCP: Research Challenge Program Role:PI Completed

Investigators:Terence L. Kirley 1995 -1996 URC: University Research Council Role:PI Completed

Investigators:Terence L. Kirley 1996 -1999 National AHA Grant-In-Aid (96013960) Role:PI Completed

Investigators:Terence L. Kirley 1999 -2001 Ohio Valley Affiliate, AHA Grant-In-Aid (9951504V) Role:PI Completed

Investigators:Terence L. Kirley and Dale W. Schaefer 07-01-2011 -12-31-2012 University of Cincinnati University Research Council An Enzyme-Immobilization Approach to Improving Surface Biocompatibility Role:Co-PI 25,000 Active Type:Grant Level:University

Grant: #R01 HL072382 Investigators:Kirley, Terence 07-14-2003 -05-31-2009 National Heart, Lung and Blood Institute Expression and Structure of Soluble Human Nucleotides Role:PI $1,452,978.00 Closed Level:Federal

Grant: #NIDA 1U01DA039550 Investigators:Andrew B. Norman, PI BAD DATE -07-31-2018 NIH Advancing the Development of a Humanized Anti-cocaine Monoclonal Antibody Role:Collborator 6,378,002 Active Type:Grant Level:National

Grant: #9951504V-01-A0-S0-E0 Investigators:Kirley, Terence 07-01-1999 -06-30-2000 American Heart Association - Southwest Chapter Active Site Determination of Human CD39 Ecto-APYrase Role:PI $34,984.00 Closed Level:Private Non-Profit

Grant: #5-R01-HL-59915-05-A0-S0-E0 Investigators:Kirley, Terence 02-01-2000 -01-31-2006 National Heart, Lung and Blood Institute Active Site Determination of the E-Type ATPases Role:PI $1,324,620.00 Closed Level:Federal

Grant: #U01DA048525 Investigators:Dasenbrock-Gamm, Hanna; Kirley, Terence; Nelson, Erik; Norman, Andrew; Ridgway, William; Webster, Rose; Welge, Jeffrey 07-01-2019 -04-30-2022 National Institute on Drug Abuse First-In-Human Study of a Humanized Anti-Cocaine Monoclonal Antibody Role:Collaborator $4,770,702.00 Awarded Level:Federal

Grant: #URC Interdisciplinary 2012-13 Investigators:Kirley, Terence 05-01-2013 -05-31-2014 UC's University Research Council Probenecid Reduces Pannexin Atp Release in Heart Failure Role:PI $25,000.00 Active Level:Internal UC

Investigators:Kirley, T.L. 07-2014 -07-2014 UC Faculty Development Council Attending Purines 2014 Conference to enhance purinergic signalling curriculum and research Role:PI Active

Grant: #NIH NIDA 1DP1DA031386 Investigators:Norman, A. B., Ball, W.J. 09-30-2010 -07-31-2015 NIH A human antibody as an immunotherapy for cocaine abuse Role:Co-Investigator 2,500,000 Active Type:Grant

Grant: #U01DA050330 Investigators:Amlal, Hassane; Crutchfield, Christopher; Dasenbrock-Gamm, Hanna; Kirley, Terence; Norman, Andrew; Rubinstein, Jack; Webster, Rose 09-15-2019 -07-31-2022 National Institute on Drug Abuse IND-Enabling Pre-Clinical Studies to Accelerate the Clinical Development of a Humanized Anti-Cocaine Monoclonal Antibody Role:Collaborator $1,796,316.00 Awarded Level:Federal

Grant: #U01DA039550 Investigators:Ball, William; Kirley, Terence; Lorenz, John; Norman, Andrew; Tsibulsky, Vladimir 08-01-2015 -05-31-2020 National Institute on Drug Abuse Advancing the Development of a Humanized Anti-cocaine Monoclonal Antibody Role:Collaborator 6259187.00 Closed Level:Federal

Grant: #1U01DA039550-01 Investigators:Dr. Andrew B. Norman, PI BAD DATE -07-31-2018 NIH NIDA grant 1U01DA039550-01 Advancing the Development of a Humanized Anti-cocaine Monoclonal Antibody Role:Co-Investigator 4,043,748 Active Type:Grant

Grant: #U01DA050330 Investigators:Amlal, Hassane; Crutchfield, Christopher; Kirley, Terence; Norman, Andrew; Rubinstein, Jack; Webster, Rose; Wetzel, Hanna 09-15-2019 -07-31-2024 National Institute on Drug Abuse IND-Enabling Pre-Clinical Studies to Accelerate the Clinical Development of a Humanized Anti-Cocaine Monoclonal Antibody Role:Collaborator 0.00 Awarded Level:Federal

Published Abstracts

H.B. Halsall, T.L. Kirley and E.D. Sprague (1980. ) Preparation and binding of spin labeled steroids to orosomucoid .[Abstract]Fed. Proc., 39 ,1906

E.D. Sprague, T.L. Kirley and H.B. Halsall (1982. ) Propranolol and progesterone binding to orosomucoid .[Abstract]Biophys. J., 37 ,383a

T.L. Kirley and H.B. Halsall (1982. ) The thermal stability of orosomucoid .[Abstract]Biophys J., 37 ,390a

H.B. Halsall, T.L. Kirley, J.A. Wallace, E.D. Sprague and M.L. Friedman (1983. ) Steroid and drug binding to human orosomucoid .[Abstract]Fed. Proc., 42 ,2102

T.L. Kirley and A. Schwartz (1984. ) 3H-Nimodipine and 3H-nitrendipine binding to rabbit skeletal t-tubular membranes: effects of d-cis-diltiazem and attempts to solubilize and affinity label the putative calcium channel .[Abstract]Biophys. J., 45 ,396a

T.L. Kirley, E.T. Wallick, T. Wang and L.K. Lane (1985. ) Species and tissue comparisons of the amino acid sequence of the ATP binding sites of (Na,K)-ATPase and Ca-ATPase .[Abstract]Biophys. J., 47 ,182a

T.L. Kirley, L.K. Lane and E.T. Wallick (1985. ) Effect of modification of tyrosine residues on (Na,K)-ATPase by tetranitromethane .[Abstract]Fed. Proc., 44 ,1172

T.L. Kirley (1986. ) Inhibition of the rabbit skeletal muscle transverse tubule Mg-ATPase by diethylpyrocarbonate .[Abstract]Biophys. J., 49 ,554a

T.L. Kirley An improved method for electroblotting of protein from SDS-PAGE gels for direct microsequencing .[Abstract]Proc. of the Sixth International Conference on Methods in Protein Sequence Analysis,

T.L. Kirley (1988. ) Purification and characterization of the Mg-ATPase from rabbit skeletal muscle .[Abstract]Biophys. J., 53 ,486a

T.L. Kirley (1988. ) Identification of three disulfide bonds and one free sulfhdryl in the ß subunit of (Na,K)-ATPase .[Abstract]Biophys. J., 55 ,425a

E. McKenna, P.L. Vaghy, K. Naito, K. Itagaki, T.L. Kirley and A. Schwartz (1989. ) Analysis of the putative subunits of a voltage-dependent calcium channel .[Abstract]Biophys. J., 55 ,301a

T.L. Kirley (1990. ) Examination of possible subunit structure of the transverse tubule Mg-ATPase .[Abstract]Biophys. J., 57 ,38a

H.R. Hinz and T.L. Kirley (1990. ) Identification of a reactive lys residue in the putative ATP binding site of lamb kidney (Na,K)-ATPase .[Abstract]Biophys. J., 57 ,356a

G. Kelso, T. L. Kirley and J.A.K. Harmony (1990. ) Analysis of disulfide bonds within the novel Apolipoprotein J. isolated from human plasma .[Abstract]Fourth Symposium of the Protein Society, San Diego, CA,

D.A. Cox, P.T. Ellinor, W. Koch, T. Kirley and M.A. Matlib (1990. ) Identification of the peripheral-type benzodiazepine binding site (PBS) in vascular smooth muscle .[Abstract]ASPET,

M. Treuheit and T.L. Kirley (1991. ) Structures of the complex glycans found in the ß-subunit of Na,K-ATPase .[Abstract]Glycoconjugate J., 8 ,258-259

T.L. Kirley (1991. ) The transverse tubule Mg-ATPase is a highly glycosylated multiple subunit enzyme .[Abstract]Biophys. J., 59 ,113a

M.J. Treuheit and T.L. Kirley (1992. ) The transverse tubule Mg-ATPase is a 70 kDa glycoprotein .[Abstract]Biophys. J., 61 ,A117 (675)

C.H. Pedemonte, J.H. Kaplan, M.J. Treuheit and T.L. Kirley (1992. ) Inactivation and the labeling of the (Na,K)-ATPase by SITS and H2-DIDS .[Abstract]Biophys. J., 61 ,A121 (702)

T.L. Kirley and M.J. Treuheit (1992. ) Purification of rabbit skeletal muscle transverse tubule magnesium ATPase for structural analyses .[Abstract]Sixth Symposium of the Protein Society, T87 ,

M.J. Treuheit, C.E. Costello and T.L. Kirley (1993. ) Structures of the complex glycans found on the ß-subunits of lamb and dog kidney (Na,K)-ATPase .[Abstract]Biophys. J., 64 ,A371

M.J. Treuheit and T.L. Kirley (1993. ) Reversibility of Cysteine Labeling by 4-(Aminosulfonyl)-7-Fluoro-2,1,3-Benzoxadiazole (ABD-F) .[Abstract]Protein Society Meeting,

T.L. Kirley (1993. ) Purification of Mg-ATPase of Chicken Gizzard and Heart - Comparison to the Rabbit Skeletal Enzyme .[Abstract]Protein Society Meeting,

E.T.Wallick, T.L.Kirley, M.J.Treuheit and A. Ataei (1993. ) Purification and characterization of the alpha and beta subunits of Na,K-ATPase .[Abstract]Protein Society Meeting,

J.G. Stout and T.L. Kirley (1994. ) Characterization of Chicken Muscle Ecto-Mg-ATPases .[Abstract]Biophys. J., 66 ,A118

M. Peng, R. Diebold, H. Fan, A. Caswell, T.L. Kirley, J. Wang and A. Schwartz (1994. ) Isolation and Tissue Distribution of Three Triadin Isoforms in Rabbit Skeletal Muscle .[Abstract]Biophys. J., 66 ,

J. G. Stout, A. Brittsan and T.L. Kirley (1994. ) Brain Mg-ATPase is Not the Neural Cell Adhesion Molecule .[Abstract]Protein Society Meeting,

T.L. Kirley and J. G. Stout (1996. ) Fragmentation, cDNA Cloning and Sequencing of the Chicken Muscle ecto-ATPase .[Abstract]First International Workshop on Ecto-ATPases,

T.L. Kirley, R. S. Strobel and J. G. Stout (1996. ) Purification and Characterization of the Chicken Gizzard (smooth muscle) ecto-ATPase .[Abstract]First International Workshop on Ecto-ATPases,

T.L. Kirley and J. G. Stout (1996. ) Ecto-ATPase Activity is Controlled by Oligomerization State .[Abstract]First International Workshop on Ecto-ATPases,

T.M. Smith, T.L. Kirley and T.M. Hennessey (1996. ) Purification and Characterization of a Soluble Ecto-ATPase from Tetrahymena Thermophilia .[Abstract]First International Workshop on Ecto-ATPases,

T.L. Kirley and T. M. Smith (1999. ) cDNA cloning, sequencing, and expression of an ecto-apyrase from human brain: Importance of glycosylation and oligomerization .[Abstract]Second International Workshop on Ecto-ATPases and Related Ectonucleotidases,

T. M. Smith and T.L. Kirley (1999. ) Site-directed mutagenesis of an E-type ATPase: Relatedness to the actin/HSP 70/sugar kinase superfamily and conversion of an ecto-apyrase to an ecto-NTPase .[Abstract]Second International Workshop on Ecto-ATPases and Related Ectonucleotidases,

C. Hicks-Berger, B. P. Chadwick, A-M Frischauf and T. L. Kirley (2000. ) Expression and Initial Characterization of CD39L2 Ecto-nucleotidase: a Soluble GDPase .[Abstract]ASBMB/ASPET meeting, Boston MA,

Sevigny J., JA. Dranoff, N. Braun, R. Lemmens, E. Kruglov, E. Csizmadia, R.N. Smith, H. Zimmermann, T.L. Kirley and S.C. Robson (2001. ) Identification and characterization of membrane-associated hepatic NTP diphosphohydrolases. 52nd Annual Meeting of the American Association for the Study of Liver Diseases (AASLD), Dallas, TX, USA. Hepathology, 34 ,451A

D.M. Murphy, V.V. Ivanenkov, F. Yang, C.A. Hicks-Berger, T.M. Smith and T.L. Kirley (2002. ) Site-directed mutagenesis of cell membrane NTPDases: Implications for structure and function of the ecto-apyrases and ecto-ATPases .[Abstract]The 7th International Symposium on Adenosine & Adenine Nucleotides, Gold Coast, Australia,

D.M. Murphy, V.V. Ivanenkov and T.L. Kirley (2002. ) Structure-Function of Nucleotide Triphosphate Diphosphohydrolases: Mutagenesis of Membranous NTPDase3 and Bacterial Expression and Characterization of Soluble NTPDase6 .[Abstract]The 3rd International Workshop on Ecto-ATPases and Related Ecto-Nucleotidases, Woods Hole, MA,

D.M. Murphy and T.L. Kirley (2002. ) Site-Directed Mutagenesis of a Highly Conserved Glycosylation Site in the ACR1 region of Human Nucleoside Triphosphate Diphosphohydrolase-3 .[Abstract]The 3rd International Workshop on Ecto-ATPases and Related Ecto-Nucleotidases, Woods Hole, MA,

V.V. Ivanenkov and T.L. Kirley (2004. ) Characterization of disulfide bonds and inter-subunit contacts in human nucleoside triphosphate diphosphohydrolase 3 (NTPDase3) .[Abstract]4th International Symposium of Nucleosides and Nucleotides (Purines 2004), Chapel Hill, N.C.,

T.L. Kirley (2004. ) Structure-function studies on nucleoside triphosphate diphosphohydrolases (NTPDases) .[Abstract]4th International Symposium of Nucleosides and Nucleotides (Purines 2004), Chapel Hill, N.C.,

T.L. Kirley, A. Zsarnovszky, P.A. Crawford, L. Spurling and S.M. Belcher (2005. ) Immunolocalization of ecto-nucleoside triphosphate diphosphohydrolase 3 (NTPDase3) in rat brain: Implications for modulation of multiple homeostatic systems including feeding and sleep-wake behaviors .[Abstract]Neuroscience Meeting 2005, Washington, D.C., November 2005,

T. L. Kirley (2006. ) Progress on the Structures and Functions of Nucleoside Triphosphate Diphosphohydrolases and a Calcium Activated Nucleotidase .[Abstract]8th International Adenosine and Adenine Nucleotides Symposium, Ferrara, Italy, May 2006,

J. Lecka, M.M. Munkonda, M. Fausther, É.G. Lavoie, G. Kauffenstein, S.A. Levesque, I. Kogan, J. Pelletier, A. Tramblay, M.S. Rana, V.V. Ivanenkov, T.L. Kirley, B. Fischer, and J. Sévigny (2008. ) Specific NTPDase Inhibitors .[Abstract]Purines 2008, Copenhagen, Denmark,

M.M. Munkonda, M. Fausther, V.V. Ivanenkov, J. Pelletier, A. Tremblay, B. Künzli, T.L. Kirley, and J. Sévigny (2008. ) Generation and characterization of a monoclonal antibody as the first specific inhibitor of human NTPDase3 .[Abstract]Purines 2008, Copenhagen, Denmark,

T.L. Kirley, W.L. Seibel, and M.D. Wortman (2014. ) Small molecule inhibitors of Ca-activated and NTPDase classes of nucleotidases .[Abstract]Purines 2014 international meeting, Invited Speaker, Bonn, Germany, July, 2014,

Peer Reviewed Publications

Gaddie, Keith J; Kirley, Terence L (2010. ) Proline residues link the active site to transmembrane domain movements in human nucleoside triphosphate diphosphohydrolase 3 (NTPDase3).Purinergic signalling, , 6 (3 ) ,327-37 More Information

Ivanenkov, Vasily V; Crawford, Patrick A; Toyama, Aimi; Sévigny, Jean; Kirley, Terence L (2010. ) Epitope mapping in cell surface proteins by site-directed masking: defining the structural elements of NTPDase3 inhibition by a monoclonal antibody.Protein engineering, design & selection : PEDS, , 23 (7 ) ,579-88 More Information

Gaddie, Keith J; Kirley, Terence L (2009. ) Conserved polar residues stabilize transmembrane domains and promote oligomerization in human nucleoside triphosphate diphosphohydrolase 3.Biochemistry, , 48 (40 ) ,9437-47 More Information

Munkonda, Mercedes N; Pelletier, Julie; Ivanenkov, Vasily V; Fausther, Michel; Tremblay, Alain; Künzli, Beat; Kirley, Terence L; Sévigny, Jean (2009. ) Characterization of a monoclonal antibody as the first specific inhibitor of human NTP diphosphohydrolase-3 : partial characterization of the inhibitory epitope and potential applications.The FEBS journal, , 276 (2 ) ,479-96 More Information

Ivanenkov, Vasily V; Sévigny, Jean; Kirley, Terence L (2008. ) Trafficking and intracellular ATPase activity of human ecto-nucleotidase NTPDase3 and the effect of ER-targeted NTPDase3 on protein folding.Biochemistry, , 47 (35 ) ,9184-97 More Information

Yang, Mingyan; Horii, Katsunori; Herr, Andrew B; Kirley, Terence L (2008. ) Characterization and importance of the dimer interface of human calcium-activated nucleotidase.Biochemistry, , 47 (2 ) ,771-8 More Information

Yang, Mingyan; Kirley, Terence L (2008. ) Engineered human soluble calcium-activated nucleotidase inhibits coagulation in vitro and thrombosis in vivo.Thrombosis research, , 122 (4 ) ,541-8 More Information

Mingyan Yang, Katsunori Horii, Andrew B. Herr, and Terence L. Kirley (2008. ) Characterization and importance of the dimer interface of human calcium-activated nucleotidase .Biochemistry, , 47 ,771-778

Kittel, Agnes; Sperlágh, Beata; Pelletier, Julie; Sévigny, Jean; Kirley, Terence L (2007. ) Transient changes in the localization and activity of ecto-nucleotidases in rat hippocampus following lipopolysaccharide treatment.International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, , 25 (5 ) ,275-82 More Information

Crawford, Patrick A; Gaddie, Keith J; Smith, Thomas M; Kirley, Terence L (2007. ) Characterization of an alternative splice variant of human nucleoside triphosphate diphosphohydrolase 3 (NTPDase3): a possible modulator of nucleotidase activity and purinergic signaling.Archives of biochemistry and biophysics, , 457 (1 ) ,7-15 More Information

Yang, Mingyan; Horii, Katsunori; Herr, Andrew B; Kirley, Terence L (2006. ) Calcium-dependent dimerization of human soluble calcium activated nucleotidase: characterization of the dimer interface.The Journal of biological chemistry, , 281 (38 ) ,28307-17 More Information

Smith, Thomas M; Kirley, Terence L (2006. ) The calcium activated nucleotidases: A diverse family of soluble and membrane associated nucleotide hydrolyzing enzymes.Purinergic signalling, , 2 (2 ) ,327-33 More Information

Kirley, Terence L; Crawford, Patrick A; Smith, Thomas M (2006. ) The structure of the nucleoside triphosphate diphosphohydrolases (NTPDases) as revealed by mutagenic and computational modeling analyses.Purinergic signalling, , 2 (2 ) ,379-89 More Information

Belcher, S M; Zsarnovszky, A; Crawford, P A; Hemani, H; Spurling, L; Kirley, T L (2006. ) Immunolocalization of ecto-nucleoside triphosphate diphosphohydrolase 3 in rat brain: implications for modulation of multiple homeostatic systems including feeding and sleep-wake behaviors.Neuroscience, , 137 (4 ) ,1331-46 More Information

T.L. Kirley (2006. ) Nucleoside triphosphate diphosphohydrolase-3 (NTPDase3).AfCS-Nature Molecule Pages, , More Information

T.M. Smith and T.L. Kirley (2006. ) The calcium activated nucleotidases: a diverse family of soluble and membrane associated nucleotide hydrolyzing enzymes .Purinergic Signalling, , 2 ,327-333

T.L. Kirley, P.A. Crawford, and T.M. Smith (2006. ) The structure of the nucleoside triphosphate diphosphohydrolases (NTPDases) as revealed by mutagenic and computational modeling analyses .Purinergic Signalling, , 2 ,379-389

M. Yang, K. Horii, A.B. Herr, and T.L. Kirley (2006. ) Calcium-dependent dimerization of human soluble calcium activated nucleotidase: characterization of the dimer interface .J. Biol. Chem., , 281 ,28307-28317

D.M. Murphy-Piedmonte, P.A. Crawford and T.L. Kirley (2005. ) Bacterial Expression, Folding, Purification and Characterization of Soluble NTPDase5 (CD39L4) Ecto-nucleotidase .Biochim. Biophys. Acta., , 1747 ,251-259

F. Kukulski, S. A. Lévesque, É. G. Lavoie, J. Lecka, F. Bigonnesse, A. F. Knowles, S. C. Robson, T. L. Kirley and J. Sévigny (2005. ) Comparative hydrolysis of P2 receptor agonists by NTPDases 1, 2, 3 and 8 .Purinergic Signalling, , 1 ,193-204

V.V. Ivanenkov, J. Meller and T.L. Kirley (2005. ) Characterization of Disulfide Bonds in Human Nucleoside Triphosphate Diphosphohydrolase 3 (NTPDase3): Implications for NTPDase Structural Modeling .Biochemistry, , 44 ,8998-9012

Basu, Saswata; Kirley, Terence L (2005. ) Identification of a tyrosine residue responsible for N-acetylimidazole-induced increase of activity of ecto-nucleoside triphosphate diphosphohydrolase 3.Purinergic signalling, , 1 (3 ) ,271-80 More Information

Ivanenkov, Vasily V; Meller, Jarek; Kirley, Terence L (2005. ) Characterization of disulfide bonds in human nucleoside triphosphate diphosphohydrolase 3 (NTPDase3): implications for NTPDase structural modeling.Biochemistry, , 44 (25 ) ,8998-9012 More Information

Kukulski, F; Lévesque, S A; Lavoie, E G; Lecka, J; Bigonnesse, F; Knowles, A F; Robson, S C; Kirley, T L; Sévigny, J (2005. ) Comparative hydrolysis of P2 receptor agonists by NTPDases 1, 2, 3 and 8.Purinergic signalling, , 1 (2 ) ,193-204 More Information

Murphy-Piedmonte, Deirdre M; Crawford, Patrick A; Kirley, Terence L (2005. ) Bacterial expression, folding, purification and characterization of soluble NTPDase5 (CD39L4) ecto-nucleotidase.Biochimica et biophysica acta, , 1747 (2 ) ,251-9 More Information

Basu, Saswata; Murphy-Piedmonte, Deirdre M; Kirley, Terence L (2004. ) Conserved lysine 79 is important for activity of ecto-nucleoside triphosphate diphosphohydrolase 3 (NTPDase3).Purinergic signalling, , 1 (1 ) ,51-8 More Information

Yang, Mingyan; Kirley, Terence L (2004. ) Site-directed mutagenesis of human soluble calcium-activated nucleotidase 1 (hSCAN-1): identification of residues essential for enzyme activity and the Ca(2+)-induced conformational change.Biochemistry, , 43 (28 ) ,9185-94 More Information

D.M. Murphy, V.V. Ivanenkov and T.L. Kirley (2003. ) Bacterial expression and characterization of a novel, soluble, calcium binding and calcium activated human nucleotidase .Biochemistry, , 42 ,2412-2421

D.M. Murphy and T.L. Kirley (2003. ) Asparagine 81, An Invariant Glycosylation Site Near Apyrase Conserved Region 1, is Essential for Full Enzymatic Activity of Ecto Nucleoside Triphosphate Diphosphohydrolase 3 .Arch. Biochem. Biophys., , 413 ,107-115

Ivanenkov, Vasily V; Murphy-Piedmonte, Deirdre M; Kirley, Terence L (2003. ) Bacterial expression, characterization, and disulfide bond determination of soluble human NTPDase6 (CD39L2) nucleotidase: implications for structure and function.Biochemistry, , 42 (40 ) ,11726-35 More Information

Murphy, Deirdre M; Kirley, Terence L (2003. ) Asparagine 81, an invariant glycosylation site near apyrase conserved region 1, is essential for full enzymatic activity of ecto-nucleoside triphosphate diphosphohydrolase 3. Archives of biochemistry and biophysics, , 413 (1 ) ,107-15

Murphy, Deirdre M; Ivanenkov, Vasily V; Kirley, Terence L (2003. ) Bacterial expression and characterization of a novel, soluble, calcium-binding, and calcium-activated human nucleotidase.Biochemistry, , 42 (8 ) ,2412-21 More Information

Smith, Thomas M; Hicks-Berger, Carrie A; Kim, Sunkyu; Kirley, Terence L (2002. ) Cloning, expression, and characterization of a soluble calcium-activated nucleotidase, a human enzyme belonging to a new family of extracellular nucleotidases. Archives of biochemistry and biophysics, , 406 (1 ) ,105-15

Murphy, Deirdre M; Ivanenkov, Vasily V; Kirley, Terence L (2002. ) Identification of cysteine residues responsible for oxidative cross-linking and chemical inhibition of human nucleoside-triphosphate diphosphohydrolase 3.The Journal of biological chemistry, , 277 (8 ) ,6162-9 More Information

E. Wood, M.J. Broekman, T.L. Kirley, S. Diani-Moore, M. Tickner, J.H.F. Drosopoulos, N. Islam, J.I. Park, A.J. Marcus and A.B. Rifkind (2002. ) Cell-type specificity of ectonucleotidase expression and upregulation by 2,3,7,8-tetrachlorodibenzo-p-dioxin .Arch. Biochem. Biophys., , 407 ,49-62

Kirley, T L; Yang, F; Ivanenkov, V V (2001. ) Site-directed mutagenesis of human nucleoside triphosphate diphosphohydrolase 3: the importance of conserved glycine residues and the identification of additional conserved protein motifs in eNTPDases.Archives of biochemistry and biophysics, , 395 (1 ) ,94-102 More Information

Hicks-Berger, C A; Yang, F; Smith, T M; Kirley, T L (2001. ) The importance of histidine residues in human ecto-nucleoside triphosphate diphosphohydrolase-3 as determined by site-directed mutagenesis. Biochimica et biophysica acta, , 1547 (1 ) ,72-81

Yang, F; Hicks-Berger, C A; Smith, T M; Kirley, T L (2001. ) Site-directed mutagenesis of human nucleoside triphosphate diphosphohydrolase 3: the importance of residues in the apyrase conserved regions. Biochemistry, , 40 (13 ) ,3943-50

Hicks-Berger, C A; Chadwick, B P; Frischauf, A M; Kirley, T L (2000. ) Expression and characterization of soluble and membrane-bound human nucleoside triphosphate diphosphohydrolase 6 (CD39L2).The Journal of biological chemistry, , 275 (44 ) ,34041-5 More Information

Hicks-Berger, C A; Kirley, T L (2000. ) Expression and characterization of human ecto-ATPase and chimeras with CD39 ecto-apyrase.IUBMB life, , 50 (1 ) ,43-50 More Information

Kirley, T L; Gerber, L K; Smith, T M (1999. ) Expression and characterization of chicken muscle ecto-ATPase in mammalian COS cells.IUBMB life, , 48 (1 ) ,67-72 More Information

Smith, T M; Lewis Carl, S A; Kirley, T L (1999. ) Mutagenesis of two conserved tryptophan residues of the E-type ATPases: inactivation and conversion of an ecto-apyrase to an ecto-NTPase.Biochemistry, , 38 (18 ) ,5849-57 More Information

Smith, T M; Kirley, T L (1999. ) Glycosylation is essential for functional expression of a human brain ecto-apyrase.Biochemistry, , 38 (5 ) ,1509-16 More Information

Smith, T M; Kirley, T L (1999. ) Site-directed mutagenesis of a human brain ecto-apyrase: evidence that the E-type ATPases are related to the actin/heat shock 70/sugar kinase superfamily.Biochemistry, , 38 (1 ) ,321-8 More Information

Smith, T M; Carl, S A; Kirley, T L (1998. ) Immunological detection of ecto-ATPase in chicken and rat tissues: characterization, distribution, and a cautionary note. Biochemistry and molecular biology international, , 45 (5 ) ,1057-66

Lewis Carl, S A; Smith, T M; Kirley, T L (1998. ) Cross-linking induces homodimer formation and inhibits enzymatic activity of chicken stomach ecto-apyrase. Biochemistry and molecular biology international, , 44 (3 ) ,463-70

T.M. Smith and T.L. Kirley (1998. ) Cloning, sequencing and expression of a human brain ecto-apyrase related to both the ecto-ATPases and CD39 ecto-apyrases .Biochem. Biophys. Acta, , 1386 ,65-78

Lewis-Carl, S; Kirley, T L (1997. ) Immunolocalization of the ecto-ATPase and ecto-apyrase in chicken gizzard and stomach. Purification and N-terminal sequence of the stomach ecto-apyrase. The Journal of biological chemistry, , 272 (38 ) ,23645-52

Dombrowski, K E; Brewer, K A; Maleckar, J R; Kirley, T L; Thomas, J W; Kapp, J A (1997. ) Identification and partial characterization of ectoATPase expressed by immortalized B lymphocytes.Archives of biochemistry and biophysics, , 340 (1 ) ,10-8 More Information

Smith, T M; Kirley, T L; Hennessey, T M (1997. ) A soluble ecto-ATPase from Tetrahymena thermophila: purification and similarity to the membrane-bound ecto-ATPase of smooth muscle. Archives of biochemistry and biophysics, , 337 (2 ) ,351-9

Kirley, T L (1997. ) Complementary DNA cloning and sequencing of the chicken muscle ecto-ATPase. Homology with the lymphoid cell activation antigen CD39. The Journal of biological chemistry, , 272 (2 ) ,1076-81

Stout, J G; Kirley, T L (1996. ) Control of cell membrane ecto-ATPase by oligomerization state: intermolecular cross-linking modulates ATPase activity.Biochemistry, , 35 (25 ) ,8289-98 More Information

Stout, J G; Kirley, T L (1995. ) Inhibition of purified chicken gizzard smooth muscle ecto-ATPAse by P2 purinoceptor antagonists. Biochemistry and molecular biology international, , 36 (5 ) ,927-34

Stout, J G; Strobel, R S; Kirley, T L (1995. ) Identification and immunolocalization of ecto-ATPDase in chicken stomach. Biochemistry and molecular biology international, , 36 (3 ) ,529-35

Stout, J G; Strobel, R S; Kirley, T L (1995. ) Properties of and proteins associated with the extracellular ATPase of chicken gizzard smooth muscle. A monoclonal antibody study. The Journal of biological chemistry, , 270 (20 ) ,11845-50

Stout, J G; Brittsan, A; Kirley, T L (1994. ) Brain ECTO-Mg-ATPase is not the neural cell adhesion molecule. Biochemistry and molecular biology international, , 33 (6 ) ,1091-8

Peng, M; Fan, H; Kirley, T L; Caswell, A H; Schwartz, A (1994. ) Structural diversity of triadin in skeletal muscle and evidence of its existence in heart. FEBS letters, , 348 (1 ) ,17-20

Stout, J G; Kirley, T L (1994. ) Purification and characterization of the ecto-Mg-ATPase of chicken gizzard smooth muscle. Journal of biochemical and biophysical methods, , 29 (1 ) ,61-75

Stout, J G; Kirley, T L (1994. ) Tissue distribution of ecto-Mg-ATPase in adult and embryonic chicken. Biochemistry and molecular biology international, , 32 (4 ) ,745-53

Treuheit, M J; Ataei, A; Wallick, E T; Kirley, T L (1993. ) Purification of the alpha and beta subunits of (Na,K)-ATPase by continuous elution electrophoresis. Preparative biochemistry, , 23 (3 ) ,375-87

Webster, Rose P; Cohen, Cinder F; Saeed, Fatima O; Wetzel, Hanna N; Ball, William J; Kirley, Terence L; Norman, Andrew B (2018. ) Evaluation of methods to reduce background using the Python-based ELISA_QC program.Journal of immunological methods, , More Information

Treuheit, M J; Costello, C E; Kirley, T L (1993. ) Structures of the complex glycans found on the beta-subunit of (Na,K)-ATPase. The Journal of biological chemistry, , 268 (19 ) ,13914-9

Treuheit, M J; Kirley, T L (1993. ) Reversibility of cysteine labeling by 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole.Analytical biochemistry, , 212 (1 ) ,138-42 More Information

Wood, Emily; Broekman, M Johan; Kirley, Terence L; Diani-Moore, Silvia; Tickner, Michelle; Drosopoulos, Joan H F; Islam, Naziba; Park, Joshua I; Marcus, Aaron J; Rifkind, Arleen B (2002. ) Cell-type specificity of ectonucleotidase expression and upregulation by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Archives of biochemistry and biophysics, , 407 (1 ) ,49-62

Pedemonte, C H; Kirley, T L; Treuheit, M J; Kaplan, J H (1992. ) Inactivation of the Na,K-ATPase by modification of Lys-501 with 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid (SITS). FEBS letters, , 314 (1 ) ,97-100

Smith, T M; Kirley, T L (1998. ) Cloning, sequencing, and expression of a human brain ecto-apyrase related to both the ecto-ATPases and CD39 ecto-apyrases1. Biochimica et biophysica acta, , 1386 (1 ) ,65-78

Treuheit, M J; Vaghy, P L; Kirley, T L (1992. ) Mg(2+)-ATPase from rabbit skeletal muscle transverse tubules is 67-kilodalton glycoprotein. The Journal of biological chemistry, , 267 (17 ) ,11777-82

Kirley, Terence L; Greis, Kenneth D; Norman, Andrew B (2018. ) Domain unfolding of monoclonal antibody fragments revealed by non-reducing SDS-PAGE.Biochemistry and biophysics reports, , 16 ,138-144 More Information

Kirley, Terence L; Norman, Andrew B (2018. ) Unfolding of IgG domains detected by non-reducing SDS-PAGE.Biochemical and biophysical research communications, , 503 (2 ) ,944-949 More Information

M.J. Treuheit, A. Ataei, T.L. Kirley and E.T. Wallick (1992. ) Purification of the glycosylated ß-subunit of the (Na,K)-ATPase by lectin affinity chromatography .Chromatographia, , 33 ,521-524

Kirley T.L.;Greis K.D.;Norman A.B. (12-01-2018. ) Domain unfolding of monoclonal antibody fragments revealed by non-reducing SDS-PAGE.Biochemistry and Biophysics Reports, , 16 ,138-144 More Information

Webster R.P.;Cohen C.F.;Saeed F.O.;Wetzel H.N.;Ball W.J.;Kirley T.L.;Norman A.B. (05-01-2018. ) Evaluation of methods to reduce background using the Python-based ELISA_QC program.Journal of Immunological Methods, , 456 ,61-66 More Information

Chai Q.;Huang Y.;Kirley T.L.;Ayres N. (09-14-2017. ) Shape memory polymer foams prepared from a heparin-inspired polyurethane/urea.Polymer Chemistry, , 8 (34 ) ,5039-5048 More Information

Argekar S.;Kirley T.;Schaefer D. (04-28-2013. ) Determination of structure-property relationships for 3- aminopropyltriethoxysilane films using x-ra.Journal of Materials Research, , 28 (8 ) ,1118-1128 More Information

Gaddie K.;Kirley T. (03-29-2010. ) Proline residues link the active site to transmembrane domain movements in human nucleoside triphosp.Purinergic Signalling, , 6 (3 ) ,327-337 More Information

Kirley, T L; Peng, M (1991. ) Identification of cysteine residues in lamb kidney (Na,K)-ATPase essential for ouabain binding. The Journal of biological chemistry, , 266 (30 ) ,19953-7

Smith T.;Kirley T. (05-01-2006. ) The calcium activated nucleotidases: A diverse family of soluble and membrane associated nucleotide .Purinergic Signalling, , 2 (2 ) ,327-333 More Information

Kirley, T L (1991. ) The Mg(2+)-ATPase of rabbit skeletal-muscle transverse tubule is a highly glycosylated multiple-subunit enzyme. The Biochemical journal, , 278 ( Pt 2) ,375-80

Kirley T.;Crawford P.;Smith T. (05-01-2006. ) The structure of the nucleoside triphosphate diphosphohydrolases (NTPDases) as revealed by mutagenic.Purinergic Signalling, , 2 (2 ) ,379-389 More Information

Cox, D A; Ellinor, P T; Kirley, T L; Matlib, M A (1991. ) Identification of a 17-kDa protein associated with the peripheral-type benzodiazepine receptor in vascular and other smooth muscle types. The Journal of pharmacology and experimental therapeutics, , 258 (2 ) ,702-9

Basu S.;Kirley T. (09-01-2005. ) Identification of a tyrosine residue responsible for N- acetylimidazole-induced increase of activity.Purinergic Signalling, , 1 (3 ) ,271-280 More Information

Hinz, H R; Kirley, T L (1990. ) Lysine 480 is an essential residue in the putative ATP site of lamb kidney (Na,K)-ATPase. Identification of the pyridoxal 5'-diphospho-5'-adenosine and pyridoxal phosphate reactive residue. The Journal of biological chemistry, , 265 (18 ) ,10260-5

Kukulski F.;Levesque S.;Lavoie E.;Lecka J.;Bigonnesse F.;Knowles A.;Robson S.;Kirley T.;Sevigny J. (09-01-2005. ) Erratum: Comparative hrydrolysis of P2 receptor agonists by NTPDases 1, 2, 3 and 8. (Purinergic Sign.Purinergic Signalling, , 1 (3 ) ,293 More Information

Kirley, T L (1990. ) Inactivation of (Na+,K+)-ATPase by beta-mercaptoethanol. Differential sensitivity to reduction of the three beta subunit disulfide bonds. The Journal of biological chemistry, , 265 (8 ) ,4227-32

Kukulski F.;Lévesque S.;Lavoie E.;Lecka J.;Bigonnesse F.;Knowles A.;Robson S.;Kirley T.;Sévigny J. (06-01-2005. ) Comparative hydrolysis of P2 receptor agonists by NTPDases 1, 2, 3 and 8.Purinergic Signalling, , 1 (2 ) ,193-204 More Information

Basu S.;Murphy-Piedmonte D.;Kirley T. (12-01-2004. ) Conserved lysine 79 is important for activity of ecto-nucleoside triphosphate diphosphohydrolase 3 (.Purinergic Signalling, , 1 (1 ) ,51-58 More Information

Murphy D.;Kirley T. (01-01-2003. ) Erratum: Asparagine 81, an invariant glycosylation site near apyrase conserved region 1, is essentia.Archives of Biochemistry and Biophysics, , 419 (2 ) ,251-252 More Information

Treuheit M.;Kirley T. (01-01-1994. ) Instability of Cysteine Labeling With 4-(aminosulfonyl)-7-fluoro-2, 1, 3-benzoxadiazole (ABD-F).Techniques in Protein Chemistry, , 5 (C ) ,189-194 More Information

Treuheit M.J.;Kirley T.L. (01-01-1993. ) Reversibility of cysteine labeling by 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole.Analytical Biochemistry, , 212 (1 ) ,138-142 More Information

Treuheit M.;Ataei A.;Kirley T.;Wallick E. (06-01-1992. ) Purification of the glycosylated beta subunit of (Na,K)-ATPase by lectin affinity chromatography.Chromatographia, , 33 (11-12 ) ,521-524 More Information

Kirley, T L (1989. ) Reduction and fluorescent labeling of cyst(e)ine-containing proteins for subsequent structural analyses. Analytical biochemistry, , 180 (2 ) ,231-6

Kirley T.;Lane L.;Wallick E. (01-01-1985. ) Effect of modification of tyrosine residues on (Na,K)-ATPase by tetranitromethane .Federation Proceedings, , 44 (3 ) ,

Kirley, T L (1989. ) Determination of three disulfide bonds and one free sulfhydryl in the beta subunit of (Na,K)-ATPase. The Journal of biological chemistry, , 264 (13 ) ,7185-92

Sprague E.;Kirley T.;Halsall H. (01-01-1982. ) Propranolol and progesterone binding to orosomucoid .Biophysical Journal, , 37 (2 II ) ,

Tyson, P A; Steinberg, M; Wallick, E T; Kirley, T L (1989. ) Identification of the 5-iodoacetamidofluorescein reporter site on the Na,K-ATPase. The Journal of biological chemistry, , 264 (2 ) ,726-34

Kirley, Terence L; Norman, Andrew B (2020. ) Ligand binding to a humanized anti-cocaine mAb detected by non-reducing SDS-PAGE.Biochemistry and biophysics reports, , 23 ,100795 More Information

Kirley, T L (1988. ) Purification and characterization of the Mg2+-ATPase from rabbit skeletal muscle transverse tubule. The Journal of biological chemistry, , 263 (25 ) ,12682-9

Gallagher, Zahra J; Fleetwood, Sara; Kirley, Terence L; Shaw, Maureen A; Mullins, Eric S; Ayres, Neil; Foster, E Johan (2020. ) Heparin Mimic Material Derived from Cellulose Nanocrystals.Biomacromolecules, , 21 (3 ) ,1103-1111 More Information

Ball, W J; Kirley, T L; Lane, L K (1988. ) Preparation of antibodies to Na+,K+-ATPase and its subunits. Methods in enzymology, , 156 ,87-101

Kirley, Terence L; Norman, Andrew B; Wetzel, Hanna N (2020. ) A novel differential scanning fluorimetry analysis of a humanized anti-cocaine mAb and its ligand binding characteristics.Journal of immunological methods, , 476 ,112676 More Information

Steinberg, M; Tyson, P A; Wallick, E T; Kirley, T L (1988. ) Identification and localization of the 5-iodoacetamido-fluorescein reporter site on dog kidney Na,K-ATPase. Progress in clinical and biological research, , 273 ,39-44

Tan, Kemin; Zhou, Min; Ahrendt, Angela J; Duke, Norma E C; Tabaja, Nassif; Ball, William J; Kirley, Terence L; Norman, Andrew B; Joachimiak, Andrzej; Schiffer, Marianne; Wilton, Rosemarie; Pokkuluri, P Raj (2019. ) Structural analysis of free and liganded forms of the Fab fragment of a high-affinity anti-cocaine antibody, h2E2.Acta crystallographica. Section F, Structural biology communications, , 75 (Pt 11 ) ,697-706 More Information

Kirley, Terence L; Norman, Andrew B (2018. ) Unfolding of IgG domains detected by non-reducing SDS-PAGE.Biochemical and biophysical research communications, , 503 (2 ) ,944-949 More Information

Kirley, Terence L; Norman, Andrew B (2015. ) Characterization of a recombinant humanized anti-cocaine monoclonal antibody and its Fab fragment.Human vaccines & immunotherapeutics, , 11 (2 ) ,458-67 More Information

Kirley T.L.;Norman A.B. (09-01-2020. ) Ligand binding to a humanized anti-cocaine mAb detected by non-reducing SDS-PAGE.Biochemistry and Biophysics Reports, , 23 , More Information

Nakayama, N; Kirley, T L; Vaghy, P L; McKenna, E; Schwartz, A (1987. ) Purification of putative Ca2+ channel protein from rabbit skeletal muscle. Determination of the amino-terminal sequence. The Journal of biological chemistry, , 262 (14 ) ,6572-6

Kirley, Terence L; Norman, Andrew B (2021. ) Ligand binding to a humanized anti-cocaine mAb measured by dye absorption spectroscopy.Biochemical and biophysical research communications, , 535 ,93-98 More Information

Lane, L K; Kirley, T L; Ball, W J (1986. ) Structural studies on H+,K+-ATPase: determination of the NH2-terminal amino acid sequence and immunological cross-reactivity with Na+,K+-ATPase. Biochemical and biophysical research communications, , 138 (1 ) ,185-92

Kirley, Terence L; Norman, Andrew B (2020. ) Multi-domain unfolding of the Fab fragment of a humanized anti-cocaine mAb characterized by non-reducing SDS-PAGE.Biochemical and biophysical research communications, , 533 (3 ) ,580-585 More Information

Kirley, T L; Lane, L K; Wallick, E T (1986. ) Identification of an essential sulfhydryl group in the ouabain binding site of (Na,K)-ATPase. The Journal of biological chemistry, , 261 (10 ) ,4525-8

Kirley, Terence L; Norman, Andrew B (2021. ) Cocaine binding to the Fab fragment of a humanized anti-cocaine mAb quantitated by dye absorption and fluorescence spectroscopy.Journal of immunological methods, , 496 ,113103 More Information

Kirley, Terence L; Norman, Andrew B (2022. ) Critical evaluation of fluorescent dyes to evaluate the stability and ligand binding properties of an anti-cocaine mAb, h2E2.Journal of immunological methods, , 113323 More Information

Kirley, Terence L; Greis, Kenneth D; Norman, Andrew B (2022. ) Tyrosine nitration of a humanized anti-cocaine mAb differentially affects ligand binding of cocaine and its metabolites.Biochemistry and biophysics reports, , 30 ,101278 More Information

Kirley, Terence L; Norman, Andrew B; Greis, Kenneth D (2022. ) Oxidation of specific tryptophan residues inhibits high-affinity binding of cocaine and its metabolites to a humanized anticocaine mAb.The Journal of biological chemistry, , 298 (3 ) ,101689 More Information

Kirley, Terence L; Norman, Andrew B (2023. ) Reformulation and Thermal Stability of a Therapeutic Anti-Cocaine mAb.Journal of pharmaceutical sciences, , More Information

Friedman, M L; Schlueter, K T; Kirley, T L; Halsall, H B (1985. ) Fluorescence quenching of human orosomucoid. Accessibility to drugs and small quenching agents. The Biochemical journal, , 232 (3 ) ,863-7

Kirley, Terence L; Norman, Andrew B (2022. ) Isothermal titration calorimetry determination of thermodynamics of binding of cocaine and its metabolites to humanized h2E2 anti-cocaine mAb.Biochemistry and biophysics reports, , 32 ,101354 More Information

Kirley, Terence L; Norman, Andrew B (2023. ) Characterization and optimization of fluorescein isothiocyanate labeling of humanized h2E2 anti-cocaine mAb.Biochemistry and biophysics reports, , 35 ,101520 More Information

Kirley, T L; Wang, T; Wallick, E T; Lane, L K (1985. ) Homology of ATP binding sites from Ca2+ and (Na,K)-ATPases: comparison of the amino acid sequences of fluorescein isothiocyanate labeled peptides. Biochemical and biophysical research communications, , 130 (2 ) ,732-8

Kirley, T L; Wallick, E T; Lane, L K (1984. ) The amino acid sequence of the fluorescein isothiocyanate reactive site of lamb and rat kidney Na+- and K+-dependent ATPase. Biochemical and biophysical research communications, , 125 (2 ) ,767-73

Kirley, T L; Schwartz, A (1984. ) Solubilization and affinity labeling of a dihydropyridine binding site from skeletal muscle: effects of temperature and diltiazem on [3H]dihydropyridine binding to transverse tubules. Biochemical and biophysical research communications, , 123 (1 ) ,41-9

Halsall, H B; Kirley, T L (1982. ) The denaturation of orosomucoid. Archives of biochemistry and biophysics, , 216 (2 ) ,392-9

Kirley, T L; Sprague, E D; Halsall, H B (1982. ) The binding of spin-labeled propranolol and spin-labeled progesterone by orosomucoid. Biophysical chemistry, , 15 (3 ) ,209-16

Kirley, T L; Halsall, H B (1982. ) Facile synthesis of spin labelled steroids by esterification. Journal of steroid biochemistry, , 16 (1 ) ,133-4

Halsall, H B; Kirley, T L; Friedman, M L (1982. ) The preparation of orosomucoid from nephrotic urine.Preparative biochemistry, , 12 (2 ) ,111-20 More Information

Halsall, H B; Kirley, T L (1981. ) Observations on the thermostability of orosomucoid. Biochemical and biophysical research communications, , 102 (2 ) ,761-5

S.U. Argekar, T.L. Kirley, D.W. Schaefer (2013. ) Determination of Structure-Property Relationships for 3-aminopropyltriethoxysilane (APTES) Films Using X-Ray Reflectivity .Journal of Materials Research, , 28 (8 ) ,1118-1128

Kirley, Terence L; Norman, Andrew B (2015. ) Characterization of a recombinant humanized anti-cocaine monoclonal antibody and its Fab fragment.Human vaccines & immunotherapeutics, , 11 (2 ) ,458-67 More Information

Huang, Yongshun; Shaw, Maureen A; Mullins, Eric S; Kirley, Terence L; Ayres, Neil (2014. ) Synthesis and anticoagulant activity of polyureas containing sulfated carbohydrates.Biomacromolecules, , 15 (12 ) ,4455-66 More Information

Wetzel, Hanna N; Webster, Rose P; Saeed, Fatima O; Kirley, Terence L; Ball, William J; Norman, Andrew B (2017. ) Characterization of a recombinant humanized anti-cocaine monoclonal antibody produced from multiple clones for the selection of a master cell bank candidate.Biochemical and biophysical research communications, , 487 (3 ) ,690-694 More Information

Kirley, Terence L; Greis, Kenneth D; Norman, Andrew B (2016. ) Selective disulfide reduction for labeling and enhancement of Fab antibody fragments.Biochemical and biophysical research communications, , 480 (4 ) ,752-757 More Information

Kirley, Terence L; Greis, Kenneth D; Norman, Andrew B (2016. ) Structural characterization of expressed monoclonal antibodies by single sample mass spectral analysis after IdeS proteolysis.Biochemical and biophysical research communications, , 477 (3 ) ,363-8 More Information

Contreras, Christopher J; Segvich, Dyann M; Mahalingan, Krishna; Chikwana, Vimbai M; Kirley, Terence L; Hurley, Thomas D; DePaoli-Roach, Anna A; Roach, Peter J (2016. ) Incorporation of phosphate into glycogen by glycogen synthase.Archives of biochemistry and biophysics, , 597 ,21-9 More Information

Q. Chai, Y. Huang, T. L. Kirley and N. Ayres (2017. ) Shape memory polymer foams prepared from a heparin-inspired polyurethane/urea.Polymer Chemistry, , 8 ,5039-5048 More Information

Invited Publications

T.L. Kirley, E.T. Wallick and L.K. Lane (1985. ) Isolation, characterization and sequencing of the putative ATP binding site of rat and lamb (Na,K)-ATPase .The Sodium Pump, 4th International Conference on Na,K-ATPase, 101-107

E.T. Wallick, T.L. Kirley and A. Schwartz Structural studies on the cardiac glycoside receptor .Cardiac Glycosides 1785-1985, Biochemistry, Pharmacology - Clinical Relevance, 27-33

T.L. Kirley (1987. ) An improved method for blotting protein from SDS-PAGE cells for direct microsequencing .Methods in Protein Sequence Analysis 1986, 303-309

W.J. Ball, T.L. Kirley and L.K. Lane (1988. ) Preparation of antibodies to Na,K-ATPase and its subunits .Methods in Enzymology, 156: Biomembranes, Part P ,87-101

M. Steinberg, P.A. Tyson, E.T. Wallick and T.L. Kirley (1988. ) Identification and localization of the 5-Iodoacetamido-fluorescein reporter site on dog kidney Na,K-ATPase .The Ion Pumps: Structure, Function and Regulation,

G.J. Kelso, T.L. Kirley and J.A.K. Harmony (1991. ) Analysis of disulfide bonds within the novel apolipoprotein J isolated from human plasma .Techniques in Protein Chemistry II, 305-312

T.L. Kirley and H.R. Hinz (1991. ) Lysine 480 is an essential residue in the putative ATP binding site of lamb kidney (Na,K)-ATPase ."The Sodium Pump: Recent Developments", 4th Annual Symposium of the Society of General Physiologists, Volume 46, Part 2 ,205-209

T.L. Kirley (1991. ) Disulfide bonds in the (Na,K)-ATPase -- are they important? ."The Sodium Pump: Recent Developments", 4th Annual Symposium of the Society of General Physiologists, Volume 46, Part 2 ,211-215

M.J. Treuheit and T. L. Kirley (1994. ) Instability of Cysteine Labeling with 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F) ."Techniques in Protein Chemistry V", 189-194

T.L. Kirley and J.G. Stout (1997. ) Purification, Characterization, and Molecular Cloning of the Chicken Gizzard Smooth Muscle ecto- ATPase .“Ecto-ATPases: Recent Progress on Structure and Function”, Proceedings of the First International Workshop on Ecto-ATPases, 111-126

T.M. Smith, M.Y. Kim, T.L. Kirley and T.M. Hennessey (1997. ) Ecto-ATPase of Tetrahymena: Role in Purinergic Responses and Purification of a Soluble Form .“Ecto-ATPases: Recent Progress on Structure and Function”, Proceedings of The First International Workshop on Ecto-ATPases, 135-142

T.M. Smith and T.L. Kirley (2000. ) Expression, Characterization, and Site-Directed Mutagenesis of a Human E-Type ATPase .The Proceedings of the Second International Workshop on Ecto-ATPases and Related Ectonucleotidases,

H. Zimmermann, A.R. Beaudoin, M. Bollen, J.W. Goding; G. Guidotti, T.L. Kirley, S.C. Robson and K. Sano (2000. ) Proposed nomenclature for two novel nucleotide hydrolyzing enzyme families expressed on the cell surface .The Proceedings of the Second International Workshop on Ecto-ATPases and Related Ectonucleotidases,

Published Books

L. Plesner, T.L. Kirley, and A.F. Knowles (1997. ) Ecto-ATPases: Recent Progress on Structure and Function .New York, New York , Plenum Press (Co-Editor)

Lecture

Terence L Kirley (1987. ) Methods in Experimental Pharmacology .

Terence L Kirley (1988. )

Terence L Kirley

Terence L Kirley (1990. )

Terence L Kirley (1990. )

Terence L Kirley (1995. )

Terence L Kirley (1996. )

Terence L Kirley (1997. )

Terence L Kirley (1997. )

Terence L Kirley (1999. )

Terence L Kirley

Terence L Kirley (2007. )

Terence L Kirley (1999. )

Terence L Kirley (2008. )

1991 -1996 NIH Research Career Development Award

12-1985 University Research Council Faculty Research Support

1981 -1982 University Research Council Summer Fellowship

1981 Laws Fellowship

1979 Henry Hochstetter Prize For excellence as a teaching assistant

1978 -1979 Teaching Assistantship

1978 -1982 University Graduate Scholarship

1978 Graduated Summa Cum Laude

1974 -1978 National Merit Finalist and Scholarship

1974 -1976 Louise Semple Taft Classics Scholarship

(Protein Chemistry Core Facility, Director ) 1985 -1993

(Protein Chemistry Service Center, Director ) 1985 -1997

(Departmental Faculty Forum, Representative ) 1986 -1989

(Faculty Forum ) Secretary 1987 -1988

(Faculty Leave Committee ) 1989 -1992

(Departmental Seminar Director ) 1990 -1992

(East Campus Smoking Policy Implementation Committee )

(Departmental Graduate Education Committee ) 1993 -To Present

(Graduate Student Research Forum Poster Competition Judge ) 1991 -1995

(Departmental Faculty Workload Policy Committee ) Committee Chair 1993 -1994

("Task Force for Pharmacology" Committee ) 02-1994 -04-1994

(Pharmacology Chair Search Committee ) 1995 -1996

(Pharmacology Medical Education Committee ) 1995 -1996

(Pharmacology Departmental Space Committee ) 1996 -To Present

(Pharmacology Departmental Space Committee Co-Chair ) 1996 -2003

(Pharmacology Departmental Space Committee ) Committee Chair 2003 -2007

(Pharmacology Department Graduate Director ) 1996 -1999

(College of Medicine Graduate Education Committee ) 1996 -1999

(Pharmacology Departmental ARPT Committee ) 1990 -1993

(Pharmacology Departmental ARPT Committee ) 1997 -2001

(Pharmacology Departmental ARPT Committee ) 2002 -2006

(University of Cincinnati Electronic Dissertation Committee ) 1998 -2000

(Pharmacology Faculty Search Committee ) 1999 -2001

(All-University Graduate Faculty member ) 2000 -To Present

(Departmental Research Enhancement Committee ) 2001 -2007

(Pharmacology Business Manager Search Committee ) 04-2003 -12-2003

(Pharmacology Departmental ARPT Committee ) Committee Chair 2003 -2005

(PCB Proteomics facility Director ) 2006 -2007

(MSB Indoor Environmental Working Group ) 01-2006 -05-2006

(Medical Pharmacology Course Assistant Director ) 2006 -2007

(Year II Promotions Board Committee ) 2006 -2007

(Medical Pharmacology Course Steering Committee ) 2007 -To Present

(Departmental Graduate Curriculum Committee ) Committee Chair 2007 -To Present

(Endowed Chair search, “Catalysis”, UC Chemistry Department External Member ) 2007 -To Present

(Medical Pharmacology Course Co-Director ) 2008 -2012

(• Pharmacology- Pathology Merger Feasibility Committee ) Committee Member Type:Departmental Service Level:Department 10-2008 -10-2009

(Basic Science Integration sub-taskforce of the Curriculum Analysis and Revision Taskforce (CART) for the College of Medicine ) Committee Member Type:University/College Service Level:College 05-2010 -07-2011

(• Fundamentals Block sub-taskforce of the CART for the College of Medicine ) Committee Member Type:University/College Service Level:College 09-2010 -07-2011

(Member of the UCCOM 2011 LCME Accreditation Self-Study Task Force ) Committee Member Type:University/College Service Level:College 11-2010 -07-2011

(Member of the AAUP Contract mandated Joint Committee on COM Issues ) Committee Member Type:University/College Service Level:University 07-2011 -04-2013

(Member of the Class of 2015 Medical School Promotion Board (PAC) ) Committee Member Type:University/College Service Level:College 10-2011 -06-2015

(M1/M2 Implementation Curriculum Committee ) Committee Member Type:University/College Service Level:College 09-2012 -To Present

(Departmental PhD graduate program website re-organization and design ) Committee Chair Type:Departmental Service Level:Department 10-2012 -12-2012

(COM Undergrad Minor in Medical Sciences, Course Director and Steering Committee member ) Committee Member Type:University/College Service Level:College 01-2013 -To Present

(Medical Education Multi-Systems Block Content Expert Group ) Committee Member Type:University/College Service Level:College 07-2013 -03-2014

(M1/M2 Medical School Curriculum Committee ) Committee Member Type:University/College Service Level:College 08-2013 -To Present

(COM Undergraduate Major, Working Groups for Curriculum and Resources ) Committee Member Type:University/College Service Level:College 01-2014 -03-2014

(M1/M2 Pharmacology content oversight in Medical Education Blocks ) Committee Chair Type:University/College Service Level:College 2013 -To Present

(M1/M2 Pharmacology in Medical Education Sub-Committee ) Committee Chair Type:University/College Service Level:College 05-2014 -To Present

Antibodies,Biochemistry,cDNA Cloning and Sequencing,Chromatography,ecto-nucleotiodase,HPLC,Mechanism of Drug Action,Membrane Transport,Molecular Biology,Peptide and Protein Sequencing,Pharmacologic Chemistry,Pharmacology,Protein Chemistry,Protein Purification,Protein Structure,Purinergic signaling,Receptor,Site of Drug Action,Site-directed Mutagenesis

Academic - Cardiovascular Center
Room 5926
Cincinnati  Ohio, 45267-0575
Phone: 513-558-2353
terry.kirley@uc.edu