Xuefeng Wang

My laboratory is dedicated to biomedical studies with innovative biophysical and biochemical approaches. Our current research focuses on the mechanobiology and enzymology of blood cells including platelets, monocytes/macrophages and neutrophils. We develop state-of-art DNA-based biosensors to visualize and map the forces and DNase activities generated by these cells, and investigate the physiological functions of these cellular forces and membrane-bound DNases during hemostasis and innate immune response.

Professional Experience
Associate Professor, Hoxworth blood center, UC, 08/2023 – Present
Associate Professor, Iowa State University, 01/2020 – 07/2023
Assistant Professor, Iowa State University, 08/2015 – 12/2019
Postdoctoral Fellow, UIUC, 05/2010 – 07/2015
Ph.D. Purdue University, 09/2004 – 12/2009
M.S. Tsinghua University, 09/2001 – 07/2004
B.S. Tsinghua University, 09/1997 – 09/2001

Grant: #R35GM128747 Investigators:Wang, Xuefeng 08-01-2023 -07-31-2024 National Institute of General Medical Sciences Study the role of integrin tension in cell migration, platelet contraction and invadopodium dynamics Role:PI 405000.00 Awarded Level:Federal

Grant: #026400A / ECCS-2204447 Investigators:Wang, Xuefeng 03-01-2024 -12-31-2025 National Science Foundation Platelets on Chip: Studies of Mechanobiology of Platelet-Mediated Thrombosis Enabled by Molecular Fluorescence Sensors Grafted inside Microfluidic Chips Role:PI 98415.00 Hold Level:Federal

Grant: #2R35GM128747-07 Investigators:Wang, Xuefeng 01-01-2025 -12-31-2029 National Institute of General Medical Sciences Study the role of integrin tension in cell migration, platelet contraction and invadopodium dynamics Role:PI 0.00 Hold Level:Federal

Peer Reviewed Publications

J. Austin, Y. Tu, K. Pal and X. Wang (2023. ) Vinculin transmits high-level integrin tensions which are dispensable for focal adhesion formation .Biophysical Journal, ,

Y. Tu, K. Pal, J. Austin and X. Wang (2022. ) Filopodial adhesive force in discrete nodes revealed by integrin molecular tension imaging .Current Biology, ,

K. Pal, Y. Tu and X. Wang (2022. ) Single-molecule force imaging reveals that podosome formation requires no extracellular integrin-ligand tensions or interactions .ACS Nano, ,

Y. Tu and X. Wang (2021. ) Tracking cell migration by cellular force footprint recorded with a mechano-optical biosensor .Biosensors and Bioelectronics, ,

S. Mao, A. Sarkar, Y. Wang, C. Song, D. N. LeVine, X. Wang and L. Que (2021. ) Microfluidic chip grafted with integrin tension sensors for evaluating the effects of flowing shear stress and ROCK inhibitor on platelets .Lab on a chip, ,

K. Pal, Y. Zhao, Y. Wang and X. Wang (2021. ) Ubiquitous membrane-bound DNase activity in podosomes and invadopodia .Journal of Cell Biology, ,

Y. Tu and X. Wang (2020. ) Recent Advances in Cell Adhesive Force Microscopy .Sensors, ,

A. Sarkar, D. N. LeVine, N. Kuzmina, Y. Zhao and X. Wang (2020. ) Cell migration driven by self-generated integrin ligand gradient on ligand-labile surfaces .Current Biology, ,

Y. Zhao, K. Pal, Y. Tu and X. Wang (2020. ) Cellular force nanoscopy with 50 nm resolution based on integrin molecular tension imaging and localization .Journal of the American Chemical Society, ,

Y. Zhao, A. Sarkar and X. Wang (2020. ) Peptide nucleic acid based tension sensor for cellular force imaging with strong DNase resistance .Biosensors and Bioelectronics, ,

Y. Zhao, N. Wetter and X. Wang (2019. ) Imaging integrin tension and cellular force at submicron resolution with integrative tension sensor .Journal of Visualized Experiments, ,

12. Y. Wang, Y. Zhao, A. Sarkar, X. Wang (2019. ) Optical sensor revealed abnormal nuclease spatial activity on cancer cell membrane .Journal of Biophotonics, ,

Y. Zhao, Y. Wang, A. Sarkar and X. Wang (2018. ) Keratocytes Generate High Integrin Tension at the Trailing Edge to Mediate Rear De-adhesion during Rapid Cell Migration .iScience, ,

A. Sarkar, Y. Zhao, Y. Wang and X. Wang (2018. ) Force-activatable coating enables high-resolution cellular force imaging directly on regular cell culture surfaces .Physical Biology, ,

X. Wang, S. Park, L. Zeng, A. Jain and T. Ha (2018. ) Towards single-cell single-molecule pull-down .Biophysical Journal, ,

Y. Wang, D. N. LeVine, M. Gannon, Y. Zhao, A. Sarkar, B. Hoch and X. Wang (2018. ) Force-activatable biosensor enables single platelet force mapping directly by fluorescence imaging .Biosensors and Bioelectronics, ,

Y. Wang and X. Wang (2016. ) Integrins outside focal adhesions transmit tensions during stable cell adhesion .Scientific Reports, ,

X. Wang and T. Ha (2013. ) Defining Single Molecular Forces Required to Activate Integrin and Notch Signaling .Science, ,

A.Sarkar*, G. Niraula*, D. N. LeVine, Y. Zhao, Y. Tu, K. Mollaeian, J. Ren, L. Que and X. Wang (2023. ) Develop a ratiometric tension sensor exclusively responding to integrin tension magnitude in live cells .ACS Sensors, ,

G. Niraula*, A. Pyne* and X. Wang (2024. ) Develop Tandem Tension Sensor to Gauge Integrin-transmitted Molecular Forces .ACS Sensors, ,

Academic - Hoxworth Blood Center
Phone: 513-558-1450
Fax: 513-558-1522