William Zacharias , MD

University Hospital of Pennsylvaniz Philadelphia, PA, 2012 (Internship in Internal Medicine)

University Hospital of Pennsylvania Philadelphia, PA, 2013 (Residency in Internal Medicine)

University Hospital of Pennsylvania Philadelphia, PA, 2017 (Fellowship in Pulmonary and Critical Care Medicine)

University Hospital of Pennsylvania Philadelphia, PA, 2018 (Postdoctoral Fellow)

B.A.: Northwestern University Evanston, IL, 2003 (Biological Sciences; Departmental Honors for Research in Biology)

M.D.: Univeristy of Michigan Ann Arbor, MI, 2011 (School of Medicine, Ph.D., Dept. of Cell and Developmental Biology)

American Board of Internal Medicine (Critical Care Medicine) (Certification Date: 10-18-2017 )

American Board of Internal Medicine (Certification Date: 08-03-2015 )

American Board of Internal Medicine (Pulmonary Disease) (Certification Date: 10-05-2016 )

Critical Care Medicine

Lung Disease

Pulmonary Disease

Chronic Cough

Dyspnea

Histoplasmosis

Legionnaires' Disease (Legionellosis)

Pleurisy

Pneumonia

Respiration Disorders

Respiratory Insufficiency

Respiratory Syncytial Virus (RSV)

Respiratory Tract Disease

Respiratory Tract Infections

Severe Acute Respiratory Syndrome (SARS)

Shortness of Breath

Sin Nombre Virus Infection (Hantavirus pulmonary syndrome)

Thoracic Disease

Tuberculosis

Whooping Cough (Pertussis)

Critical Care Medicine

Internal Medicine

Grant: #K08-HL140178 07-21-2018 -06-30-2023 NIH/NHLBI Mechanistic evaluation of a novel WNT-responsive adult alveolar epithelial progenitor population during regeneration after diffuse alveolar damage Role:PI 163080 Active Type:Grant

Peer Reviewed Publications

Matsui, Satoshi; Granitto, Marissa; Buckley, Morgan; Ludwig, Katie; Koigi, Sandra; Shiley, Joseph; Zacharias, William J; Mayhew, Christopher N; Lim, Hee-Woong; Iwafuchi, Makiko (2024. ) Pioneer and PRDM transcription factors coordinate bivalent epigenetic states to safeguard cell fate.Molecular cell, , 84 (3 ) ,476-489.e10 More Information

He, Hua; Bell, Sheila M; Davis, Ashley Kuenzi; Zhao, Shuyang; Sridharan, Anusha; Na, Cheng-Lun; Guo, Minzhe; Xu, Yan; Snowball, John; Swarr, Daniel T; Zacharias, William J; Whitsett, Jeffrey A (2023. ) PRDM3/16 Regulate Chromatin Accessibility Required for NKX2-1 Mediated Alveolar Epithelial Differentiation and Function.bioRxiv : the preprint server for biology, , More Information

Toth, Andrea; Kannan, Paranthaman; Snowball, John; Kofron, Matthew; Wayman, Joseph A; Bridges, James P; Miraldi, Emily R; Swarr, Daniel; Zacharias, William J (2023. ) Alveolar epithelial progenitor cells require Nkx2-1 to maintain progenitor-specific epigenomic state during lung homeostasis and regeneration.Nature communications, , 14 (1 ) ,8452 More Information

Alarcon, Pablo C; Damen, Michelle S M A; Ulanowicz, Cassidy J; Sawada, Keisuke; Oates, Jarren R; Toth, Andrea; Wayland, Jennifer L; Chung, Hak; Stankiewicz, Traci E; Moreno-Fernandez, Maria E; Szabo, Sara; Zacharias, William J; Divanovic, Senad (2023. ) Obesity amplifies influenza virus-driven disease severity in male and female mice.Mucosal immunology, , 16 (6 ) ,843-858 More Information

Guo, Minzhe; Wikenheiser-Brokamp, Kathryn A; Kitzmiller, Joseph A; Jiang, Cheng; Wang, Guolun; Wang, Allen; Preissl, Sebastian; Hou, Xiaomeng; Buchanan, Justin; Karolak, Justyna A; Miao, Yifei; Frank, David B; Zacharias, William J; Sun, Xin; Xu, Yan; Gu, Mingxia; Stankiewicz, Pawel; Kalinichenko, Vladimir V; Wambach, Jennifer A; Whitsett, Jeffrey A (2023. ) Single Cell Multiomics Identifies Cells and Genetic Networks Underlying Alveolar Capillary Dysplasia.American journal of respiratory and critical care medicine, , 208 (6 ) ,709-725 More Information

Wilburn, Adrienne N; McAlees, Jaclyn W; Haslam, David B; Graspeuntner, Simon; Schmudde, Inken; Laumonnier, Yves; Rupp, Jan; Chougnet, Claire A; Deshmukh, Hitesh; Zacharias, William J; König, Peter; Lewkowich, Ian P (2023. ) Delayed Microbial Maturation Durably Exacerbates Th17-driven Asthma in Mice.American journal of respiratory cell and molecular biology, , 68 (5 ) ,498-510 More Information

Reza, Abid A; Kohram, Fatemeh; Reza, Hasan A; Kalin, Timothy R; Kannan, Paranthaman S; Zacharias, William J; Kalinichenko, Vladimir V (2023. ) FOXF1 Regulates Alveolar Epithelial Morphogenesis through Transcriptional Activation of Mesenchymal WNT5A.American journal of respiratory cell and molecular biology, , 68 (4 ) ,430-443 More Information

Khattar, Divya; Fernandes, Sharlene; Snowball, John; Guo, Minzhe; Gillen, Matthew C; Jain, Suchi Singh; Sinner, Debora; Zacharias, William; Swarr, Daniel T (2022. ) PI3K signaling specifies proximal-distal fate by driving a developmental gene regulatory network in SOX9+ mouse lung progenitors.eLife, , 11 , More Information

Stevens, Joseph; Steinmeyer, Shelby; Bonfield, Madeline; Peterson, Laura; Wang, Timothy; Gray, Jerilyn; Lewkowich, Ian; Xu, Yan; Du, Yina; Guo, Minzhe; Wynn, James L; Zacharias, William; Salomonis, Nathan; Miller, Lisa; Chougnet, Claire; O'Connor, Dennis Hartigan; Deshmukh, Hitesh (2022. ) The balance between protective and pathogenic immune responses to pneumonia in the neonatal lung is enforced by gut microbiota.Science translational medicine, , 14 (649 ) ,eabl3981 More Information

Jackson, Courtney M; Demmert, Martin; Mukherjee, Shibabrata; Isaacs, Travis; Thompson, Ravyn; Chastain, Chase; Gray, Jerilyn; Senthamaraikannan, Paranth; Presicce, Pietro; Chetal, Kashish; Salomonis, Nathan; Miller, Lisa A; Jobe, Alan H; Kallapur, Suhas G; Zacharias, William J; Lewkowich, Ian P; Deshmukh, Hitesh; Chougnet, Claire A (2022. ) A potent myeloid response is rapidly activated in the lungs of premature Rhesus macaques exposed to intra-uterine inflammation.Mucosal immunology, , 15 (4 ) ,730-744 More Information

Toth, Andrea; Steinmeyer, Shelby; Kannan, Paranthaman; Gray, Jerilyn; Jackson, Courtney M; Mukherjee, Shibabrata; Demmert, Martin; Sheak, Joshua R; Benson, Daniel; Kitzmiller, Joseph; Wayman, Joseph A; Presicce, Pietro; Cates, Christopher; Rubin, Rhea; Chetal, Kashish; Du, Yina; Miao, Yifei; Gu, Mingxia; Guo, Minzhe; Kalinichenko, Vladimir V; Kallapur, Suhas G; Miraldi, Emily R; Xu, Yan; Swarr, Daniel; Lewkowich, Ian; Salomonis, Nathan; Miller, Lisa; Sucre, Jennifer S; Whitsett, Jeffrey A; Chougnet, Claire A; Jobe, Alan H; Deshmukh, Hitesh; Zacharias, William J (2022. ) Inflammatory blockade prevents injury to the developing pulmonary gas exchange surface in preterm primates.Science translational medicine, , 14 (638 ) ,eabl8574 More Information

Sun, Xin; Perl, Anne-Karina; Li, Rongbo; Bell, Sheila M; Sajti, Eniko; Kalinichenko, Vladimir V; Kalin, Tanya V; Misra, Ravi S; Deshmukh, Hitesh; Clair, Geremy; Kyle, Jennifer; Crotty Alexander, Laura E; Masso-Silva, Jorge A; Kitzmiller, Joseph A; Wikenheiser-Brokamp, Kathryn A; Deutsch, Gail; Guo, Minzhe; Du, Yina; Morley, Michael P; Valdez, Michael J; Yu, Haoze V; Jin, Kang; Bardes, Eric E; Zepp, Jarod A; Neithamer, Terren; Basil, Maria C; Zacharias, William J; Verheyden, Jamie; Young, Randee; Bandyopadhyay, Gautam; Lin, Sara; Ansong, Charles; Adkins, Joshua; Salomonis, Nathan; Aronow, Bruce J; Xu, Yan; Pryhuber, Gloria; Whitsett, Jeff; Morrisey, Edward E (2022. ) A census of the lung: CellCards from LungMAP.Developmental cell, , 57 (1 ) ,112-145.e2 More Information

Cross, Gail; Ho, Jessica S Y; Zacharias, William; Jeyasekharan, Anand D; Marazzi, Ivan (2021. ) Emergency drug use in a pandemic: Harsh lessons from COVID-19.Cell, , 184 (22 ) ,5497-5500 More Information

Rindler, Tara N; Brown, Kari M; Stockman, Courtney A; van Lieshout, Laura P; Martin, Emily P; Weaver, Timothy E; Zacharias, William J; Wootton, Sarah K; Whitsett, Jeffrey A; Bridges, James P (2021. ) Efficient Transduction of Alveolar Type 2 Cells with Adeno-associated Virus for the Study of Lung Regeneration.American journal of respiratory cell and molecular biology, , 65 (1 ) ,118-121 More Information

Hynds, Robert E; Zacharias, William J; Nikolić, Marko Z; Königshoff, Melanie; Eickelberg, Oliver; Gosens, Reinoud; de Coppi, Paolo; Janes, Sam M; Morrisey, Edward; Clevers, Hans; Ryan, Amy L; Stripp, Barry R; Sun, Xin; Kim, Carla F; Lin, Qing S (2021. ) National Heart, Lung, and Blood Institute and Building Respiratory Epithelium and Tissue for Health (BREATH) Consortium Workshop Report: Moving Forward in Lung Regeneration.American journal of respiratory cell and molecular biology, , 65 (1 ) ,22-29 More Information

Zacharias, William (2021. ) Detecting oxygen changes in the lungs.Science (New York, N.Y.), , 371 (6524 ) ,32-33 More Information

Paris, Andrew J; Hayer, Katharina E; Oved, Joseph H; Avgousti, Daphne C; Toulmin, Sushila A; Zepp, Jarod A; Zacharias, William J; Katzen, Jeremy B; Basil, Maria C; Kremp, Madison M; Slamowitz, April R; Jayachandran, Sowmya; Sivakumar, Aravind; Dai, Ning; Wang, Ping; Frank, David B; Eisenlohr, Laurence C; Cantu, Edward; Beers, Michael F; Weitzman, Matthew D; Morrisey, Edward E; Worthen, G Scott (2020. ) STAT3-BDNF-TrkB signalling promotes alveolar epithelial regeneration after lung injury.Nature cell biology, , 22 (10 ) ,1197-1210 More Information

Jackson, Courtney M; Mukherjee, Shibabrata; Wilburn, Adrienne N; Cates, Chris; Lewkowich, Ian P; Deshmukh, Hitesh; Zacharias, William J; Chougnet, Claire A (2020. ) Pulmonary Consequences of Prenatal Inflammatory Exposures: Clinical Perspective and Review of Basic Immunological Mechanisms.Frontiers in immunology, , 11 ,1285 More Information

Rajaram, Sanjeeth; Canaday, Laura M; Ochayon, David E; Rangel, Kelly M; Ali, Ayad; Gyurova, Ivayla E; Krishnamurthy, Durga; Fletcher, Jonathan S; Reighard, Seth D; Cox, Andrew; Weirauch, Matthew T; Kottyan, Leah C; Deshmukh, Hitesh; Zacharias, William J; Borchers, Michael T; Waggoner, Stephen N (2020. ) The Promise and Peril of Natural Killer Cell Therapies in Pulmonary Infection.Immunity, , 52 (6 ) ,887-889 More Information

Swarr, Daniel; Putcha, Nirupama; Zacharias, William (2020. ) "PIK"ing Out New Epigenetic Markers in Lung Disease.American journal of respiratory and critical care medicine, , 201 (9 ) ,1029-1030 More Information

Oherle, Katherine; Acker, Elizabeth; Bonfield, Madeline; Wang, Timothy; Gray, Jerilyn; Lang, Ian; Bridges, James; Lewkowich, Ian; Xu, Yan; Ahlfeld, Shawn; Zacharias, William; Alenghat, Theresa; Deshmukh, Hitesh (2020. ) Insulin-like Growth Factor 1 Supports a Pulmonary Niche that Promotes Type 3 Innate Lymphoid Cell Development in Newborn Lungs.Immunity, , 52 (4 ) ,716-718 More Information

Basil, Maria C; Katzen, Jeremy; Engler, Anna E; Guo, Minzhe; Herriges, Michael J; Kathiriya, Jaymin J; Windmueller, Rebecca; Ysasi, Alexandra B; Zacharias, William J; Chapman, Hal A; Kotton, Darrell N; Rock, Jason R; Snoeck, Hans-Willem; Vunjak-Novakovic, Gordana; Whitsett, Jeffrey A; Morrisey, Edward E (2020. ) The Cellular and Physiological Basis for Lung Repair and Regeneration: Past, Present, and Future.Cell stem cell, , 26 (4 ) ,482-502 More Information

Oherle, Katherine; Acker, Elizabeth; Bonfield, Madeline; Wang, Timothy; Gray, Jerilyn; Lang, Ian; Bridges, James; Lewkowich, Ian; Xu, Yan; Ahlfeld, Shawn; Zacharias, William; Alenghat, Theresa; Deshmukh, Hitesh (2020. ) Insulin-like Growth Factor 1 Supports a Pulmonary Niche that Promotes Type 3 Innate Lymphoid Cell Development in Newborn Lungs.Immunity, , 52 (2 ) ,275-294.e9 More Information

Xiao, Deyi; Li, Xiaohong; Rouchka, Eric C; Waigel, Sabine; Zacharias, Wolfgang; McMasters, Kelly M; Hao, Hongying (2020. ) Comparative gene expression analysis in melanocytes driven by tumor cell-derived exosomes.Experimental cell research, , 386 (1 ) ,111690 More Information

Tighe, Robert M; Redente, Elizabeth F; Yu, Yen-Rei; Herold, Susanne; Sperling, Anne I; Curtis, Jeffrey L; Duggan, Ryan; Swaminathan, Suchitra; Nakano, Hideki; Zacharias, William J; Janssen, William J; Freeman, Christine M; Brinkman, Ryan R; Singer, Benjamin D; Jakubzick, Claudia V; Misharin, Alexander V (2019. ) Improving the Quality and Reproducibility of Flow Cytometry in the Lung. An Official American Thoracic Society Workshop Report.American journal of respiratory cell and molecular biology, , 61 (2 ) ,150-161 More Information

Alapati, Deepthi; Zacharias, William J; Hartman, Heather A; Rossidis, Avery C; Stratigis, John D; Ahn, Nicholas J; Coons, Barbara; Zhou, Su; Li, Hiaying; Singh, Kshitiz; Katzen, Jeremy; Tomer, Yaniv; Chadwick, Alexandra C; Musunuru, Kiran; Beers, Michael F; Morrisey, Edward E; Peranteau, William H (2019. ) In utero gene editing for monogenic lung disease.Science translational medicine, , 11 (488 ) , More Information

Frank, David B; Penkala, Ian J; Zepp, Jarod A; Sivakumar, Aravind; Linares-Saldana, Ricardo; Zacharias, William J; Stolz, Katharine G; Pankin, Josh; Lu, MinQi; Wang, Qiaohong; Babu, Apoorva; Li, Li; Zhou, Su; Morley, Michael P; Jain, Rajan; Morrisey, Edward E (2019. ) Early lineage specification defines alveolar epithelial ontogeny in the murine lung.Proceedings of the National Academy of Sciences of the United States of America, , 116 (10 ) ,4362-4371 More Information

Zacharias, William J; Frank, David B; Zepp, Jarod A; Morley, Michael P; Alkhaleel, Farrah A; Kong, Jun; Zhou, Su; Cantu, Edward; Morrisey, Edward E (2018. ) Regeneration of the lung alveolus by an evolutionarily conserved epithelial progenitor.Nature, , 555 (7695 ) ,251-255 More Information

Rossidis, Avery C; Stratigis, John D; Chadwick, Alexandra C; Hartman, Heather A; Ahn, Nicholas J; Li, Haiying; Singh, Kshitiz; Coons, Barbara E; Li, Li; Lv, Wenjian; Zoltick, Philip W; Alapati, Deepthi; Zacharias, William; Jain, Rajan; Morrisey, Edward E; Musunuru, Kiran; Peranteau, William H (2018. ) In utero CRISPR-mediated therapeutic editing of metabolic genes.Nature medicine, , 24 (10 ) ,1513-1518 More Information

Dolinay, Tamás; Aonbangkhen, Chanat; Zacharias, William; Cantu, Edward; Pogoriler, Jennifer; Stablow, Alec; Lawrence, Gladys G; Suzuki, Yoshikazu; Chenoweth, David M; Morrisey, Edward; Christie, Jason D; Beers, Michael F; Margulies, Susan S (2018. ) Protein kinase R-like endoplasmatic reticulum kinase is a mediator of stretch in ventilator-induced lung injury.Respiratory research, , 19 (1 ) ,157 More Information

Jacob, Anjali; Morley, Michael; Hawkins, Finn; McCauley, Katherine B; Jean, J C; Heins, Hillary; Na, Cheng-Lun; Weaver, Timothy E; Vedaie, Marall; Hurley, Killian; Hinds, Anne; Russo, Scott J; Kook, Seunghyi; Zacharias, William; Ochs, Matthias; Traber, Katrina; Quinton, Lee J; Crane, Ana; Davis, Brian R; White, Frances V; Wambach, Jennifer; Whitsett, Jeffrey A; Cole, F Sessions; Morrisey, Edward E; Guttentag, Susan H; Beers, Michael F; Kotton, Darrell N (2017. ) Differentiation of Human Pluripotent Stem Cells into Functional Lung Alveolar Epithelial Cells.Cell stem cell, , 21 (4 ) ,472-488.e10 More Information

Zepp, Jarod A; Zacharias, William J; Frank, David B; Cavanaugh, Christina A; Zhou, Su; Morley, Michael P; Morrisey, Edward E (2017. ) Distinct Mesenchymal Lineages and Niches Promote Epithelial Self-Renewal and Myofibrogenesis in the Lung.Cell, , 170 (6 ) ,1134-1148.e10 More Information

Paris, Andrew J; Liu, Yuhong; Mei, Junjie; Dai, Ning; Guo, Lei; Spruce, Lynn A; Hudock, Kristin M; Brenner, Jacob S; Zacharias, William J; Mei, Hankun D; Slamowitz, April R; Bhamidipati, Kartik; Beers, Michael F; Seeholzer, Steven H; Morrisey, Edward E; Worthen, G Scott (2016. ) Neutrophils promote alveolar epithelial regeneration by enhancing type II pneumocyte proliferation in a model of acid-induced acute lung injury.American journal of physiology. Lung cellular and molecular physiology, , 311 (6 ) ,L1062-L1075 More Information

Zacharias, William J; Madison, Blair B; Kretovich, Katherine E; Walton, Katherine D; Richards, Neil; Udager, Aaron M; Li, Xing; Gumucio, Deborah L (2011. ) Hedgehog signaling controls homeostasis of adult intestinal smooth muscle.Developmental biology, , 355 (1 ) ,152-62 More Information

Zacharias, William J; Li, Xing; Madison, Blair B; Kretovich, Katherine; Kao, John Y; Merchant, Juanita L; Gumucio, Deborah L (2010. ) Hedgehog is an anti-inflammatory epithelial signal for the intestinal lamina propria.Gastroenterology, , 138 (7 ) ,2368-77, 2377.e1-4 More Information

Kolterud, Asa; Grosse, Ann S; Zacharias, William J; Walton, Katherine D; Kretovich, Katherine E; Madison, Blair B; Waghray, Meghna; Ferris, Jennifer E; Hu, Chunbo; Merchant, Juanita L; Dlugosz, Andrzej A; Kottmann, Andreas H; Gumucio, Deborah L (2009. ) Paracrine Hedgehog signaling in stomach and intestine: new roles for hedgehog in gastrointestinal patterning.Gastroenterology, , 137 (2 ) ,618-28 More Information

Lees, Charlie W; Zacharias, William J; Tremelling, Mark; Noble, Colin L; Nimmo, Elaine R; Tenesa, Albert; Cornelius, Jennine; Torkvist, Leif; Kao, John; Farrington, Susan; Drummond, Hazel E; Ho, Gwo-Tzer; Arnott, Ian D R; Appelman, Henry D; Diehl, Lauri; Campbell, Harry; Dunlop, Malcolm G; Parkes, Miles; Howie, Sarah E M; Gumucio, Deborah L; Satsangi, Jack (2008. ) Analysis of germline GLI1 variation implicates hedgehog signalling in the regulation of intestinal inflammatory pathways.PLoS medicine, , 5 (12 ) ,e239 More Information

Krause, Ange; Zacharias, William; Camarata, Troy; Linkhart, Barbara; Law, Evelyn; Lischke, Antje; Miljan, Erik; Simon, Hans-Georg (2004. ) Tbx5 and Tbx4 transcription factors interact with a new chicken PDZ-LIM protein in limb and heart development.Developmental biology, , 273 (1 ) ,106-20 More Information